This short course will provide a comprehensive overview of the most often used antibodies in the differential diagnosis of common lesions/tumors involving the female genital tract. The course intends to highlight problematic aspects in the interpretation of these antibodies in specific scenarios in which the correct diagnosis is important for patient management. A broad range of antibodies will be discussed with emphasis on p16, CD10, inhibin, calretinin, WT1, CD99, c-kit, epithelial markers, OCT4, CD30, SALL4, p63, and p57 among others. Particular attention will be paid to problems in using these antibodies in isolation and emphasis will be given to the best panel of antibodies to be utilized. The course is intended for a wide audience including general pathologists, pathologists with special interest in gynecologic pathology, fellows and residents. The course will be structured based on discussion of 8 cases representing broad but common categories of lesions/tumors of the female genital tract that will lead to discussion of differential diagnosis and the use of immunohistochemistry including: 1) mesenchymal tumors of the uterus, 2) glandular proliferations of the uterus (endometrial vs endocervical and classification of the different histologic subtypes), c) sex cord stromal tumors of the ovary and mimics, d) surface epithelial tumors versus mesothelioma, e) primary versus metastatic carcinomas to the ovary, f) germ cell tumors, g) small round cell tumors involving ovary and peritoneum, and h) trophoblastic proliferations. There will be time for questions and discussion at the end of each case. At the conclusion of the course, the participants should be familiar with the main pitfalls associated with the interpretation of these antibodies; they should be able to identify the best panel of antibodies for lesions/tumors with overlapping morphologic features and interpret the results accurately in order to establish the correct diagnosis. We intend to provide virtual slides, a detailed syllabus to be given out to registrants at the meeting, as well as a PowerPoint presentation. After the meeting, registrants will be able to access after meeting material on the USCAP website.

(NEW COURSE) This course may be used for CME credits or SAM credits.