CD20 (L-26)
CD20 is a monoclonal antibody made in mice to human tonsillar (B) lymphocytes. It is present in the
majority of B cell lymphomas but may occasionally react with T cell lymphomas. We use a heat induced
epitope retrieval. The antibody works well in paraffin or B5-fixed material.
CD20 is the single most useful marker for B lymphocytes. However, it is important to recognize that
approximately 15% of all B cell lymphomas may be negative with CD20. This is particularly true in the case
of immature B cell proliferations such as ALL, and in cases of immunodeficiency related lymphomas. CD20
reacts with L & H (popcorn) cells in cases of lymphocyte predominance Hodgkin's disease, but also with a
small proportion of typical Reed-Sternberg cells in up to 40% of cases of mixed cellularity and nodular
sclerosis Hodgkin's disease.
References
- Cartun RW, Coles FB, Pastuszak WT: Utilization of monoclonal antibody L26 in the identification and
confirmation of B-cell lymphomas. A sensitive and specific marker applicable to formalin- and B-5 fixed,
paraffin-embedded tissues. Am J Pathol 1987;129:415.
- Cavey FR, Gatter KC, Raifkiaer B, et al: Immunophenotyping of non-Hodgkin's lymphomas using a panel of
antibodies on paraffin-embedded tissues. Am J Pathol 1987;129:54 (L-26 was erroneously designated as L27.)
- Coles FB, Cartrun RW, Pastuszak WT. Hodgkin's disease, lymphocyte predominant type: Immunoreactivity with
B-cell antibodies. Mod Pathol 1983;1:274.
- Lindar J, Yuling Y, Armitage JC, et al. Monoclonal antibodies marking B-cell non-Hodgkin's lymphoma in
paraffin-embedded tissue. Mod Pathol 1988;1:29.
- Norton AJ, Isaacson PC. Monoclonal antibody L26, an antibody that is restrictive with normal and neoplastic
B lymphocytes in routinely fixed and paraffin embedded tissue. J Clin Pathol 1987;40:1405.
- Orazi A, Jiang B, Lee C, English G, Cattoretti G, John K, Neiman RS. Correlation between presence of clonal
rearrangement of immunoglobulin heavy chain genes and B-cell antigen expression in Hodgkin's disease. Am J
Clin Pathol, 1995;104-413.
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