—  SHORT COURSE  —

THE VALUE OF IMMUNOHISTOCHEMISTRY
IN THE ASSESSMENT OF BONE MARROW DISORDERS

Attilio Orazi, M.D., FRCPath. and Dennis P. O'Malley, M.D.




OTHER MARKERS USED TO STUDY B-CELL PROLIFERATIONS:
CD5, CD23, CD10

Several recently available antibodies including CD5, CD23, and CD10 allow paraffin section immunohistochemistry to be used similarly to flow cytometry when classifing B-cell lymphoid proliferations involving the bone marrow. Similar results can be obtained with both B5 and formalin fixation. However, all these markers (CD10 in particular) require well-fixed tissue coupled to an adequate antigen retrieval strategy. Subtypes of malignant B-cell lymphomas composed of small lymphocytes show distinct antigenic profiles with CD5, CD23, and CD10. CD10 can also be used to identify the presence of a precursor B cell phenotype in blasts of cases of acute lymphoblastic leukemia/lymphoblastic lymphoma (Bavikatty et al, 2000).

References

  • Bavikatty et al: Anti-CD10 immunoperoxidase staining of paraffin-embedded acute leukemias: comparison with flow cytometric immunophenotyping. Hum Pathol. 2000;31:1051-4.
  • Chen et al: Classification of small B-cell lymphoid neoplasms using a paraffin section immunohistochemical panel. Appl Immunohistochem Molecul Morphol. 2000;8:1-11.
  • De Leon et al: Usefulness of an immunohistochemical panel in paraffin-embedded tissues for the differentiation of B-cell non-Hodgkin's lymphomas of small lymphocytes. Mod Pathol. 1998;11:1046-51.
  • Dorfman et al: Usefulness of a new CD5 antibody for the diagnosis of T-cell and B-cell lymphoproliferative disorders in paraffin sections. Mod Pathol. 1997;10:859-63.
  • Kumar et al: Use of CD23 (BU38) on paraffin sections in the diagnosis of small lymphocytic lymphoma and mantle cell lymphoma. Mod Pathol. 1996;9: 925-9.
  • Kurtin et al: Demonstration of distinct antigenic profiles of small B-cell lymphomas by paraffin section immunohistochemistry. Am J Clin Pathol. 1999;112:319-29.
  • Murray et al: CD23 expression in non-Hodgkin lymphoma: immunohistochemical demonstration using the antibody BU38 on paraffin sections. J Pathol. 1991;165:125-8.
  • Watson et al: Monoclonal antibodies recognizing CD5, CD10 and CD23 in formalin-fixed, paraffin-embedded tissue: production and assessment of their value in the diagnosis of small B-cell lymphoma. Histopathology. 2000; 36:145-50.