THE VALUE OF IMMUNOHISTOCHEMISTRY IN THE ASSESSMENT OF BONE MARROW DISORDERS
Attilio Orazi, M.D., FRCPath. and Dennis P. O'Malley, M.D.
The most specific marker available for identifying mantle cell lymphoma. Cyclin D1 positive immunostaining
identifies about 70% of cases of mantle cell lymphoma. Rare cases of atypical chronic B cell lymphocytic
leukemia and hairy cell leukemia can also express cyclin D1. Expression of cyclin D1 can be seen in
multiple myeloma. This protein can also be successfully demonstrated in the bone marrow by using an heat
induced epitope retrieval procedure (Vasef et al, 1997). The immunostaining is very useful in
distinguishing manlte cell lymphoma from other low grade lymmphoproliferative conditions encountered in the
Vasef et al: Cyclin D1 immunohistochemical staining is useful in distinguishing mantle cell lymphoma from
other low-grade B-cell neoplasms in bone marrow. Am J Clin Pathol. 1997;108:302-7.
Yang et al: Cyclin D1 (Bcl-1, PRAD1) protein expression in low-grade B-cell lymphomas and reactive
hyperplasia. Am J Pathol. 1994; 145:86-96.