THE VALUE OF IMMUNOHISTOCHEMISTRY
IN THE ASSESSMENT OF BONE MARROW DISORDERS
Attilio Orazi, M.D., FRCPath. and Dennis P. O'Malley, M.D.
Anti-CD15 is a monoclonal antibody made in mice against purified neutrophils from normal human peripheral
blood. It reacts against mature granulocytes and also against Hodgkin's and Reed-Sternberg cells. We use a
heat induced epitope retrieval method. The stain works well in both formalin and B5-fixed tissue and in
decalcified material. We do not find CD15 to be as valuable in the detection of granulocyte precursors as
myeloperoxidase, for it appears to be much less sensitive. It's major use, in our hands, is in the
diagnosis of Hodgkin's disease. In many cases, Reed-Sternberg cells and mononuclear Reed-Sternberg cells
are positive, except in cases of lymphocyte predominance. CD15 reacts with cases of metastatic carcinomas
(e.g. lung adenocarcinoma).
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