PROLIFERATION-ASSOCIATED MARKERS: PC10 AND MIB-1
PCNA is a monoclonal mouse antibody made against the proliferating cell nuclear antigen. It is a marker of
the active phases of the cell cycle. However, PCNA is also expressed in the nuclei of cells which are
undergoing apoptosis. PC10 is not species specific and can be used to stain PCNA in a variety of animal
tissues. PCNA requires the use of a heat-induced epitope retrieval technique and stains just as well well
both formalin fixed and B5 fixed tissues (Budke et al, Mod Pathol, 1994).
MIB-1 is a proliferation associated marker which stains cells in the S-phase of the cell cycle. It is
generally considered a more specific proliferation-associated marker than PC10 since MIB-1 does not stain
cells in apoptosis. MIB-1 requires a heat-induced retrieval technique and works well in formalin fixed
tissues. In contrast with PC-10, MIB-1 is human specific (Budke et al, Mod Pathol, 1994).
- Budke et al :Assessment of cell proliferation in paraffin sections of normal bone marrow by the
monoclonal antibodies Ki-67 and PCNA. Mod Pathol. 1994;7:860-6.
- Cattoretti et al: Proliferating normal bone marrow cells do stain for Ki-67 antigen. Br J Haematol.
- Cattoretti et al: Monoclonal antibodies against recombinant parts of the Ki-67 antigen (MIB 1 and MIB
3) detect proliferating cells in microwave-processed formalin-fixed paraffin sections. J Pathol.
- Orazi et al: Recombinant human interleukin-3 and recombinant human granulocyte-macrophage
colony-stimulating factor administered in vivo after high-dose cyclophosphamide cancer chemotherapy: effect
on hematopoiesis and microenvironment in human bone marrow. Blood. 1992;79:2610-9.
- Wilkins et al: A study of cell proliferation in formalin-fixed, wax-embedded bone marrow trephine
biopsies using the monoclonal antibody PC10, reactive with proliferating cell nuclear antigen (PCNA). J