THE VALUE OF IMMUNOHISTOCHEMISTRY IN THE ASSESSMENT OF BONE MARROW DISORDERS
Attilio Orazi, M.D., FRCPath. and Dennis P. O'Malley, M.D.
APOPTOSIS BY IN-SITU END LABELING
The rate of cell death due to apoptosis can be evaluated by a nonisotopic in situ end-labeling (ISEL)
technique applied to paraffin sections. Results from several groups including our suggest that an increased
level of intramedullary apoptosis is apparent in both patients with MDS and those with AML; those with
therapy-related disorders have the highest levels. In secondary AML and MDS cases, a correlation between
high degree of apoptosis in myeloid cells and p53 overexpression was also demonstrated (Orazi et al, 1996).
Controversial findings in lymphoid malignancy suggests that the apoptotic pathways may be different in this
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