USES AND LIMITATIONS OF ANCILLARY TECHNIQUES APPLIED TO CYTOPATHOLOGY
Jeffrey S. Ross, M.D.
USES AND LIMITATIONS IN FLOW AND IMAGE CYTOMETRY APPLIED TO CYTOPATHOLOGY SPECIMENS
As mentioned, the use of image cytometric and flow cytometric techniques to determine the proliferative
compartment of S-phase of a cycling group of cells is generally imprecise. Flow cytometry is superior to
image analysis in this regard, but is still considered an estimate of the S-phase percentage and may be
hampered in cytologic specimens featuring insufficient cellularity for statistically meaningful mathematical
calculations of S-phase. The remainder of this section is focused on the use of image cytometric and flow
cytometric methods in determining the modal DNA content of a tumor cell population or DNA ploidy.