POSTER SESSION IV Tuesday, March 9, 2004   —  1:00 - 4:30 PM Exhibit Hall B and C TECHNIQUES 193 Tumor Heterogeneity Affects the Significance of Fold-Changes Measured by Microarray Analysis (1520) M O'Sullivan, V Budhraja, Y Sadovksy, J Pfeifer - Washington University School of Medicine, St. Louis, MO 194 Targeting of Abnormal Plasma Cells with Image Analysis May Significantly Improve the Abnormal Detection Rate Determined by FISH in Multiple Myeloma (1487) S Chen, D Bouman, M Hibbard, P Cotter, KJ Bloom - US Labs, Irvine, CA 195 Image Cytometric Angiogenesis (CD31) and Lymphangiogenesis (D2-40): Correlation with VEGF Expression by Immunohistochemistry (IHC), and VEGF, VEGF-C, and VEGF-D by Quantitative Reverse Transcriptase Polymerase Chain Reaction (Q-RT-PCR) (1490) WWL Choi, AN Young, MM Lewis, D Lawson, Q Yin-Goen, G Cotsonis, C Cohen - Emory University School of Medicine; Atlanta Veterans Affairs Medical Center; School of Public Health, Atlanta, GA 196 Improved Software for Quantitative Analysis of Fluorescence Microscopy Images (1514) AK Meeker, J Zimmerman, JL Beckman-Hicks, AM De Marzo - Johns Hopkins University School of Medicine, Baltimore, MD; Vision Multimedia Technologies, Baltimore, MD 197 Pathology Education Instructional Resource Digital Library: Online Resources To Facilitate Pathology Education (1507) KN Jones, KTC Panizzi, S Hamza, PG Anderson - University of Alabama at Birmingham, Birmingham, AL 198 Identifying Alterations on Chromosome 5p in Bronchial Squamous Carcinoma In Situ by Array Comparative Genomic Hybridization (1484) TPH Buys, C Garnis, BP Coe, C MacAulay, S Lam, WL Lam - British Columbia Cancer Research Centre, Vancouver, BC, Canada 199 Automated Construction of Tissue Microarrays from Morphologically Selected Sites for Correlative Genotype / Phenotype Analysis of Breast and Prostate Cancer (1504) GH Hostetter, J Kakareka, G Salem, T Pohida, JM Trent, O-P Kallioniemi - Cancer Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD; Center for Information Technology, National Institutes of Health, Bethesda, MD; VTT Technical Research Centre of Finland, Turku, Finland; Translational Genomics Research Institute, Phoenix, AZ 200 Spectral Analysis of Normal and Malignant Microarray Tissue Sections Using a Novel Micro-Optoelectricalmechanical System (1513) M Maggioni, GL Davis, FJ Warner, DB Geshwind, AC Coppi, RR Coifman - Yale University, New Haven, CT; Plain Sight Systems, Hamden, CT 201 Manual Exfoliation of Fresh Tissue Obviates the Need for Frozen Sections for Molecular Profiling (1516) WD Mojica, L Liotta, V Espina - The State University of New York at Buffalo, Buffalo, NY; FDA-NCI Clinical Proteomics Program, National Cancer Institute, Bethesda, MD 202 Chromogenic In Situ Hybridization for the Detection of Epstein Barr Virus (EBV)-DNA in EBV-Associated Lymphoproliferative Disorders (1519) FP O'Connell, GS Pinkus, F Wang, JC Aster, JL Kutok - Brigham and Women's Hospital, Harvard Medical School, Boston, MA; National Institutes of Health 203 Dual Endogenous Enzyme Block for Use in Immunohistochemistry (1517) J Musser, A Knoll, U Lovborg, M Key, R Welcher - DakoCytomation, Carpinteria, CA 204 Novel Flow Cytometric Method of TCR Gene Rearrangement Evaluation: Clinical Validation and Correlation with PCR Molecular Analysis (1512) JM Luider, M Shepel, H Wong, I Auer - Calgary Laboratory Services, Calgary, AB, Canada; University of Calgary, Calgary, AB, Canada 205 Application of Holliday Junction Based Allele-Specific (HAS) Genotyping Platform for Detecting Two Common Mutations of Hereditary Hemochromatosis (1535) W Yang, T Yaoi, S Huang, S Hatcher, H Seet, JP Gregg - FreshGene, Inc., Concord, CA; University of California at Davis Medical Center, Sacramento, CA 206 Immunohistochemical Detection of Human Telomerase (hTERT) Compared with P504-S in Prostate Cancer (1524) AG Puebla-Mora, A Heras, AM Cano-Valdez, D Perez- Montiel, HR Dominguez-Malagon - Instituto Nacional de Cancerologia, Mexico City, D.F, Mexico 207 Quantitative Semi-Automated Image Analyis of Immunohistochemical Staining of Tissue Microarrays (1498) DA Faith, AM De Marzo - Johns Hopkins School of Medicine, Baltimore, MD 208 Tissue Microarray Software Applications (TMA-J) and Their Use at The Johns Hopkins Tissue Microrarray Facility (1497) DA Faith, J Morgan, B Razzaque, CJ Bennett, M Southerland, G March, H Fedor, AM De Marzo - Johns Hopkins School of Medicine, Baltimore, Md 209 Anti-NeuN Immunohistochemical Staining in Neuroendocrine Tumors (1494) SC Emery, N Weidner - University of California San Diego Medical Center, San Diego, CA 210 Determination of Optimal Preparation for Immunohistochemical Staining of Intermediate Filaments with KA4, 10.11, and Vimentin, Using Tissue Microarray Analysis (1483) LW Browne, YM Frutiger, CS Rangel, R Mehta, TM Grogan, S Kakar, ZL Tabatabai, RB Nagle - University of Arizona Health Sciences Center, Tucson, AZ; University of California, San Francisco, CA 211 Her-2 FISH Enumeration. Comparison of Manual to Automated Scoring with the Metacyte Scanning System (1532) T Thomson, D Phillips, E Hilland - British Columbia Cancer Agency, Vancouver, BC, Canada 212 Immunohistochemical Staining for COX-2 in Colorectal Cancer- A Comparison of Four Antibodies (1530) WM Smith, ML Prasad, S Jones, A Lehman, WL Frankel - The Ohio State University, Columbus, OH