POSTER SESSION III Tuesday, March 1, 2005   —  9:30 AM - 12:00 Noon Convention Center, Exhibit Hall B TECHNIQUES 195 High Sensitivity and Specificity of FISH in Routine Detection of t(14;18)(q32;q21) and t(11;14)(q13;q32) in Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections Using Automated Morphometric Image Analysis (1512) TS Barry, HC Hwang, LC Goldstein, H Yaziji, AW Hing, AM Gown - PhenoPath Laboratories and IMPRIS, Seattle, WA 196 High Sensitivity and Specificity of FISH in Routine Detection of t(X;18)(p11.2;q11.2) in Synovial Sarcomas (SS) Using Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections and Automated Morphometric Image Analysis (1513) TS Barry, J Terry, TO Nielsen, HC Hwang, LC Goldstein, AM Gown - PhenoPath Laboratories and IMPRIS, Seattle, WA; British Columbia Cancer Agency, Vancouver, BC, Canada 197 Slide Based, Whole Cell Her2neu FISH Amplification: Mechanisms of Expression and Correlation with Immunohistochemistry (1520) SA Dayan, JF Silverman, YL Liu, CA Smith, SE Shackney - Allegheny General Hospital, Pittsburgh, PA 198 An Approach to High Throughput Automated Multi-Color FISH Scanning of Tissue Microarrays (1557) RR Tubbs, E Swain, J Pettay, M Hartke, K Keslar, K Simmerman, MP Bronner, D Hicks, J Crowe, M Skacel - Cleveland Clinic Foundation, Cleveland, OH 199 Enzyme Metallography (EnzMet) - A Robust Detection System for High Resolution Ultrasensitive Immunohistochemistry (IHC) (1556) RR Tubbs, J Pettay, PC Roche, W Powell, RD Powell, T Grogan, JF Hainfeld - Cleveland Clinic Foundation, Cleveland, OH; Ventana Medical Systems, Inc., Tucson, AZ; Nanoprobes, Inc., Yaphank, NY 200 High-Throughput Automated Image Analysis of Multitarget Fluorescence In Situ Hybridization (FISH) Assay in Patients with Bladder Cancer and Atypical or Negative Urine Cytology (1527) JL Fine, E Swain, RR Tubbs, J Pettay, M Hartke, K Keslar, K Simmerman, M Skacel - Cleveland Clinic Foundation, Cleveland, OH 201 The Use of a Cytokeratin 7/CDX-2 Cocktail in the Work-Up of Adenocarcinoma of Unknown Origin (1516) KJ Bloom, D Helling, A Kyshtoobayeva, R Satyadev - ChromaVision Medical Systems, Irvine, CA; US Labs, Irvine, CA 202 CD117 Immunohistochemistry Tissue Microarray for Quality Assurance and Inter-Laboratory Comparison: A CAP Cell Markers Committee Study (1522) DM Dorfman, MM Bui, RR Tubbs, ED Hsi, AR Cohen, PL Fitzgibbons, MD Linden, RR Rickert, PC Roche - College of American Pathologists, Northfield, IL 203 Cocktail of Epithelial Markers Increases the Diagnostic Sensitivity for Sarcomatoid Carcinoma (1524) YM EL-Gohary, Lin, RS Saad, M Tung, YL Liu, JF Silverman - Allegheny General Hospital, Pittsburgh, PA 204 Quantitative ELISA-Like IHC (QUELI) on FFPE Tissue (1528) 205 Tissues Fixed in Stabilization Solution 'RNAlater' - Effect on Immunohistochemistry (1533) AA Jungbluth, D Kolb, SK Eastlake-Wade, KJ Busam, DB Flieder - Ludwig Institute for Cancer Research, New York, NY; Memorial Sloan-Kettering Cancer Ctr., New York, NY; Fox Chase Cancer Ctr., Philadelphia, PA 206 Application of Automated Standardized Immunohistochemistry in Formalin-Fixed Paraffin-Embedded Mouse Tissue for the Classification of Murine Lymphoid and Non-Lymphoid Hematopoietic Tumors (1536) S Kunder, J Calzada-Wack, J Mueller, L Quintanilla-Martinez - GSF-Research Center for Health and Environment, Neuherberg bei Munich, Germany 207 Spectral Imaging for Automated Analysis of Multicolor Immunohistochemical Studies in Hematopathology (1537) RM Levenson, C van der Loos, RC Braylan - CRI, Inc., Woburn, MA; Academical Medical Institute, Amsterdam, Netherlands; University of Florida, Gainesville, FL 208 Cell Clots: An Efficient and Accurate Method of Converting Cell Suspensions into Blocks for Immunohistochemical Staining (1547) A Polydorides, E Hyjek, A Chadburn - Weill Cornell Medical College, New York, NY 209 Tissue Array Construction: Pitfalls, Problems and Progress (1549) A Quinn, P Tang, Q Yang, P Bourne - University of Rochester Medical Center, Rochester, NY 210 Tissue Microarrays with Paraffin Tissue Core Biopsies of 0.43 mm in Diameter Are Technically Feasible (1558) UF Vogel, BD Bueltmann - University Clinic Eberhard-Karls-University, Tuebingen, Baden-Wuerttemberg, Germany 211 Fluorescence In Situ Hybridization of Already Stained Paraffin Sections Mounted on Uncoated Slides - What To Do? (1559) UF Vogel, BD Bueltmann - University Clinic, Eberhard-Karls-University, Tuebingen, Baden-Wuerttemberg, Germany 212 CD10 Expression in Non-Hematopoietic Malignant Neoplasms: A Diagnostic Pitfall in the Working up of Metastatic Carcinoma of Unknown Origin (1561) G Yue, YL Liu, M Tung, JF Silverman - Allegheny General Hospital, Pittsburgh, PA 213 Identification of the Relative Levels of Gene Expression of Androgen Receptor (AR) in 60 Different Tumor Types (1514) D Baunoch, MW Moore, ME Reyes, BW Tirtorahardjo, ML Opel, A Au, G Krause, A Tassin, NB Edejer, J Haut, X Ma, M Erlander - US Labs, Irvine, CA; Arcturus, Mountain View, CA 214 Paraffin Embedded Cell Lines Microarray (PECLIMA): A High-Throughput Method for Antigen Profiling of Cell Lines (1526) B Ferrer, R Bermudo, TM Thomson, J Calvo, M Soler, I Nayach, M Sanchez, E Campo, PL Fernandez - Hospital Clinico-IDIBAPS- Universidad de Barcelona, Barcelona, Spain; CSIC, Barcelona, Spain 215 The Diagnostic Utility of Immunostaining for Angiotensin Converting Enzyme (CD143) and CD4 in the Workup of Granulomatous Inflammation (1552) HK Singh, K Wilson, JW Woosley, SV Seshan, E Hjyek, V Nickeleit - The University of North Carolina, Chapel Hill, NC; East Carolina University, Greenville, NC; New York Presbyterian Hospital/Cornell Medical College, New York, NY 216 Profiler: A Powerful Web-Based Tissue Microarray Analysis System and Relational Database for Collaborative Translational Research (1535) R Kim, J Tang, S Lnu, R Shen, D Gibbs, V Mahavishnu, J Wei, CG Kleer, AM Chinnaiyan, MA Rubin - Brigham and Women's Hospital, Boston; Harvard Medical School, Boston; University of Michigan, Ann Arbor; Dana-Farber Cancer Institute, Boston