Ricardo V. Lloyd, M.D., Mayo Clinic, Rochester, MN and Arie Perry, M.D.,Washington University School of Medicine, St. Louis, MO
This course will be presented in two parts. The first part will be an overview of RNA and DNA in situ hybridization techniques using colometric and fluorescent approaches. The second part will be a case-based discussion. The ten slide-based cases will illustrate the ways in which in situ hybridization is used as an integral part of the work-up in diagnostic pathology. The application of in situ hybridization methods to solve problems arising in the daily practice of pathology will be emphasized.
Procedures for setting up an in situ hybridization laboratory with quality controls and CAP requirements for the laboratory will be discussed. New techniques such as peptide nucleic acid probes and tyramide amplification to detect low copy numbers of nucleic acids will be covered.
The course will be a practical guide for the novice, but should also appeal to pathologists with some experience with in situ hybridization procedures. A limited number of sets of glass slides will be available for advance mailing. All registrants will be mailed a CD after the meeting. A detailed syllabus will be distributed at the course.
Upon completion of the course, participants should be able to: 1) discuss the basic principles involved in setting up in situ hybridization tests, 2) correctly interpret results of hybridization analyses including false-positive and false-negative results and 3) correct problems arising during performance of in situ hybridization testing by problem solving. (Last Scheduled Presentation)