POSTER SESSION V Wednesday, February 15, 2006   —  9:30 AM - 12:00 Noon Exhibit Level TECHNIQUES 185 Identification Of Qualitative And Quantitative Alterations In The Mitochondria Of Enriched Cell Populations Between Matched Normal And Malignant Colonic Tissue (1545) WD Mojica, LA Hawthorn, BK Stefanick, X Fang - University at Buffalo; Roswell Park Cancer Institute, Buffalo, NY 186 Impact Of Pre-Analytic Factors On Stability Of Phosphoproteins: Implications For Clinical / Preclinical Biomarker Studies (1523) JD Deeds, MF Loda, R Meyer, Y Shim, JM Mosquera-Buitrago, RE Mosher - Novartis Institute for BioMedical Research, Cambridge, MA; Brigham and Women's Hospital, Boston, MA 187 Reproducibility of 2D-DIGE for Determining Differences in Protein Expression Levels in Complex, Low-Abundance Samples: Implications for Biomarker Discovery (1531) O Greengauz-Roberts, R Podulsky, H Stoppler, D Ferris, JR Lee, WS Dynan - Medical College of Georgia, Veterans Affairs Medical Center, Augusta, GA 188 Genotyping of Phenotypically Defined Cells: Enhanced ImmunoFISH Tyramide Signal Amplication (TSA) Protects Immunophenotypically Defined Populations for Gated Genotyping (1567) RR Tubbs, K Das, E Swain, R Lounsbury, JR Cook, JD Pettay, PC Roche, TM Grogan - Cleveland Clinic Foundation, Cleveland, OH; Ventana Medical Systems, Inc, Tucson, AZ 189 Developing Techniques In High-Throughput Telomere Analysis For The Study Of Prostatic Carcinogenesis (1537) AM Joshua, C Mei, I Braude, T Collins, B Vukovic, JR Srigley, AJ Evans, JA Squire - Ontario Cancer Institute - Princess Margaret Hospital, University Health Network, Toronto, ON, Canada; Le Chesnay Cedex, France; The Credit Vallay Hospital, Mississauga, ON, Canada 190 Quantitative Image Analysis-Based Prediction Of Prognosis In Early Stage Breast Cancer Using The Tripath Oncology ProEx Br System (1556) JS Ross, AB Boguniewicz, MS Ross, CE Sheehan - Albany Medical College, Albany, NY 191 Virtual Mitosis Recognition Through Digital Algorithms Improves And Standardizes Mitotic Counting In Diverse Human Neoplasms (1514) SH Barsky, A Josi, A Deshpande, S Keyhani-Rofagha, NJ Karlin, AS Gholap - The Ohio State University College of Medicine, Columbus, OH; Olive View, Sylmar, CA; BioImagene, Inc, San Mateo, CA 192 Diagnostic Fluorescence Microscopy Improved By Light Emitting Diodes (LED) Technology (1524) AP Dei Tos, M Angelini, MC GiangarP, N Baraldo, G Mazzini - Hospital of Treviso, Treviso, Italy; Fraen Corporation, Milan, Italy; IGM-CNR, Univ. of Pavia, Pavia, Italy 193 Availability and Quality of Paraffin Blocks Identified by the Shared Pathology Informatics Network (SPIN): A Multi-institutional Study (1549) AA Patel, D Gupta, D Seligson, EM Hattab, U Balis, TM Ulbright, JJ Berman, JR Gilbertson, MJ Becich, AV Parwani - Shared Pathology Informatics Network, Univ of Pittsburgh, Pittsburgh, PA; UCLA, Los Angeles, CA; Indiana Univeristy, Indianapolis, IN; Harvard Univ, Boston, MA; DCTD, NCI/NIH, Rockville, MD 194 The bDNA Assay (Quantigene TM) For Quantification Of RNA In Formalin-Fixed Tissues (1540) B Knudsen, A Allen, A Kristal, G McMaster - Fred Hutchinson Cancer Center, Seattle, WA; Genospectra, Fremont, CA 195 Application Of Array Cgh On Archival Formalin Fixed Paraffin Embedded Tissues (1535) NA Johnson, R Hamoudi, K Ichimura, L Liu, D Pearson, P Collins, M-Q DU - University of Cambridge, Cambridge, United Kingdom 196 Addressing The Issue Of Discordance Between Techniques: Analysis Of Chromosome 1 Abnormalities In Renal Oncocytoma By Conventional Cytogenetics, Fish And LOH (1551) MM Picken, B Chyna, JM Lee - Loyola University Medical Center, Maywood, IL 197 Sequential Scrubbing Technique For Combinations Of Molecular Abnormalities Identify Low Stage, Non-Small Cell Carcinoma Of The Lung That Do Not Need Chemotherapy (1536) DL Jones, JF Silverman, YL Liu, RS Saad, R Keenan, CA Smith, SE Shackney - Allegheny General Hospital, Laboratory of Cancer Cell Biology and Genetics, Pittsburgh, PA 198 Multispectral Image Analysis of Multiplexed Immunohistochemical Stains in Breast Cancer (1539) CA Kerfoot, ME Endlich, RM Levenson - US Labs, Irvine, CA; CRI, Woburn, MA 199 Silver In Situ Hybridization (SISH); Validation of a Fully Automated HER2 Staining Protocol. (1565) RR Tubbs, DG Hicks, JD Pettay, WC Powell, PC Roche, L Pang, EV Klohe, W Liu, RD Powell, JF Hainfeld, R Lounsbury, M Loftus, TM Grogan - Cleveland Clinic Foundation, Cleveland, OH; Ventana Medical Systems, Inc, Tucson, AZ; Nanoprobes, Inc, Yaphank, NY 200 Use Of Whole Slide Imaging For Primary Diagnosis In Surgical Pathology: An Intrainstitutional Validation Study (1533) J Ho, D Jukic, A Parwani, L Anthony, J Gilbertson - University of Pittsburgh Medical Center, Pittsburgh, PA 201 Fully Melting Of Tissue Microarrays Using A Stabilization Body (1569) UF Vogel, BD Bueltmann - University Clinic, Eberhard-Karls-University, Tuebingen, Baden-Wuerttemberg, Germany 202 The Effect Of Storage On The Antigen Expression Of Tissue Microarray Slides (1547) KC O'Reilly, MW Datta, AJ Kahler, MB Amin, AA Kajdacsy-Balla - Emory University Hospital, Atlanta, GA; Medical College of Wisconsin, Milwaukee, WI; University of Illinois-Chicago, Chicago, IL 203 Depositing Archived Paraffin Tissue Core Biopsies In Paraffin Tissue Microarrays By Using A Paraffin Tissue Punch With A Countersunk (1570) UF Vogel, BD Bueltmann - University Clinic, Eberhard-Karls-University, Tuebingen, Baden-Wuerttemberg, Germany 204 Effect Of Fixatives On Nuclear Protein Extraction And Proteomic Analyses Using Two-Dimensional Protein Electrophoresis (1538) P Kapur, LR Margraf, D Rakheja - University of Texas Southwestern Medical Center, Dallas, TX 205 Color Conversion of Enzyme Metallographic Signals: a New Ultrasensitive Method for Chromogenic Signal Generation (1553) RD Powell, VN Joshi, JF Hainfeld, K Das, R Lounsbury, J Pettay, RR Tubbs, R Levenson - Nanoprobes, Inc, Yaphank, NY; Cleveland Clinic Foundation, Cleveland, OH; CRI, Inc, Woburn, MA 206 Comparison of 4 Scales for Quantifying Antigen Expression in Immunoperoxidase Stains: Assessment of Pathologist Agreement (1564) LD True, S Hawley, A Herstein, JP Simko, L Fazli, J Virgin, EC Jones, BS Knudsen, AD Etzioni - University of Washington, Fred Hutchinson Cancer Center, Seattle, WA; University of California, San Francisco, CA; University of British Columbia, Vancouver, BC, Canada 207 In-situ Gene Expression Profiling in Clinical Samples by Quantum Dot Based Multiplex Hybridization (1562) E Tholouli, D Divizio, F O'Connell, D Twomey, R Levenson, JA Hoyland, JA Liu-Yin, CA Lee, TR Golub, M Loda, RJ Byers - Manchester Royal Infirmary, Manchester, United Kingdom; University of Manchester, Manchester, United Kingdom; Brigham & Women's Hospital, Dana-Farber Cancer Institute, MIT, Boston, MA; CRI, Woburn, MA