Section 8 -

Appendices Attilio Orazi Dennis P. O'Malley

Appendix "A" ANTIBODY SPECIES CLONE TREATMENT ALK-1 Mo ALK-1 PC-H-ph Bcl-2 Mo 124 PC-H-ph Bcl-6 Mo P1F6 PC-EDTA BOB-1 Rb C-20 ST CD10 Mo 270 ST CD117 Mo Ckit ST CD138 Mo B.B4 ST CD15 Mo C3D-1 ST CD1a Mo 010 ST CD20 Mo L26 ST CD21 Mo 1F8 PK CD23 Mo 1B12 PC-H-ph CD3 Rb ST CD30 Mo BerH2 ST CD31 Mo JC/70A ST CD34 Mo QBEnd10 ST CD4 Mo 1F6 PC-EDTA CD42b Mo mm2/174 ST CD43 Mo Leu22 ST CD45/LCA Mo B211+PD7 None CD45/RO Mo UCHL-1 None CD5 Mo 4C7 PC-H-ph CD56 Mo OB11 PC-EDTA CD57 Mo Leu7 ST CD68 Mo PGM1 PK CD7 Mo 272 ST CD79a Mo JCB117 ST CD8 Mo CD8/144B ST CD99 Mo 12E7 ST Collagen IV Mo CIV22 PK Cyclin D1 Mo DSC-6 PC-H-ph Cytokeratin 20 Mo Ks20.81 ST Cytokeratin 7 Mo OVL-TL PK EBV-LMP Mo CS1.4 PK EMA Mo E29 None Glycophorin A Mo Jc159 None Hairy Cell Mo DBA.44 PK Hemoglobin Rb PK IgA Rb PK Kappa Rb PK Ki-76 Mo Mib-1 ST Lambda Rb PK Lysozyme Rb PK Myeloperoxidase Rb None NGFR Mo NGER-5 ST P53 Mo DO.7 ST Pan-Cytokeratin Mo AE1/3 ST Pax-5 Mo 2.4 ST-EDTA Plasma Cell Mo VS38c ST S-100 Rb ST SMA Mo 1A4 None TdT Rb PC-H-ph TIA-1 Mo TIA-1 PK Tryptase Mo AA1 PK Ulex POLY LECTIN None vFW(FVIIIRag) Rb PK PC = Pressure Cooker ST = Steamer PK = Proteinase k H = High Appendix "B" Non-follicular hyperplasia 24 year old woman presented with asymptomatic splenomegaly on physical examination. The patient's history was notable for frequent infections and a long-standing diagnosis of Common Variable Immunodeficiency. Splenic marginal zone lymphoma A 43 year old man presented to his physician with increasing weakness, fatigue severe left upper quadrant pain. A concurrent CBC showed an elevated WBC (22 k/cumm) with several "abnormal" appearing lymphocytes. A splenectomy was performed and a 2.3 kg spleen was removed. Hairy cell leukemia A 43 year old man presented to his physician with early satiety and left upper quadrant discomfort. Physical examination revealed massive splenomegaly. A concurrent CBC showed a slightly elevated WBC (17 k/cumm), increased lymphocytes, monocytopenia and borderline neutropenia. A splenectomy was performed and a 3.1 kg spleen was removed. Hepatosplenic T-cell lymphoma A 24 year old male presented to the emergency room in acute distress. A CBC revealed profound cytopenias. The patient also had significant hepatosplenomegaly. Because of concerns of imminent splenic rupture, a splenectomy was performed. Diffuse large B-cell lymphoma A 70 year old woman presented to her physician with fever, night sweats and weight loss of 15 pounds in 2 weeks. Physical examination revealed splenic mass. A CT scan revealed a 15 cm mass in the spleen. A splenectomy was performed. Mastocytosis A 74 year old male had been "sick" for years. Symptoms included diarrhea, itching, headaches, bone pain and a feeling of fullness in the left upper quadrant. Radiographic examination revealed an enlarged spleen, with a diffusely abnormal signal. Splenectomy was performed and a 1700 g spleen was removed. Chronic idiopathic myelofibrosis A 55 year old male presented to his primary care physician with weakness, fatigue and lethargy. Physical examination revealed a spleen palpable at the umbilicus. A peripheral blood smear revealed a leukoerythroblastic appearance. Splenectomy was performed and a 4200 g spleen was removed. The gross appearance was "beefy" with focal purple nodules that looked like "plums". Acute myeloid leukemia A 58 year old female presented with acute left upper quadrant pain. The patient's history was remarkable for a previous diagnosis of CML in stable phase for the past 8 years. At time of splenectomy, the WBC was 53,000. Littoral cell angioma A 36 year old male presented to his physician for left upper quadrant pain. A diagnosis of pancreatitis was made based on laboratory and clinical findings. In addition, a CT scan was performed which revealed a splenic mass that was 10 cm in greatest diameter. A splenectomy was performed. The mass was poorly circumscribed, and tan with a 'bloody' appearance. Inflammatory pseudotumor 6 year old female was found to have a 1 cm splenic nodule on CT scan. There was an antecedent history of urinary tract infections. The mass was excised (spleen-conserving surgery). The resected tissue showed normal splenic appearance with a 1 cm central tan-white, well-circumscribed nodule.