2017 Annual Meeting

Clinical Application of Next Generation Sequencing for the Management of Patients with Solid Tumors

Room CC 221, March 6 2017, 8:00am to 12:00pm

Description

Clinical Application of Next Generation Sequencing for the Management of Patients with Solid Tumors
Monday, March 6, 2017
8:00 AM 12:00 PM
CC 221

Session Credits: 3.5 CME and SAMs

Course Director: Jeffrey S. Ross, MD, Albany Medical Center, Albany, NY and Foundation Medicine, Inc., Cambridge, MA

Course Co-Directors: Neal I. Lindeman, MD, Brigham and Women’s Hospital and Dana Farber Cancer Institute, Boston, MA
Stephen Yip, MD, PhD, University of British Columbia and Vancouver General Hospital, Vancouver, BC

Course Description:
This half-day course is designed to familiarize the registrant with both the technical aspects and clinical applications of NGS for the solid tumor cancer patient. The first section of the course is organized into a didactic series of short lectures given by the course instructors to highlight the history of clinical DNA sequencing on cancer specimens focused on the search for potential therapy targets for patients with relapsed and refractory disease. The technical section will emphasize practical issues for pathologists including the types of samples that can be used, the quantity and proportion of tumor cells needed and the pre-analytic factors that can influence NGS test results. Both formalin fixed paraffin embedded tissues and blood-based “liquid biopsies” will be considered. The remaining sections of the course will consist of a case by case analysis that includes lung cancer, breast cancer, colorectal cancer, melanoma, brain tumors and cancer of unknown primary origin.

Upon completion of this educational activity, participants should be able to:

  • Contrast the differences, advantages and disadvantages between next generation sequencing (NGS) technologies and traditional “Sanger Sequencing” methods
  • Understand the current limitations of “one off" single gene hotspot sequencing tests versus comprehensive genomic profiling by NGS
  • Contrast the advantages and disadvantages of the targeted NGS panel approach versus whole exome and whole genome sequencing
  • Understand how next generation sequencing technologies can be applied to detect all classes of genomic alterations including base substitutions, short insertions/deletions, fusions and translocations, and copy number alterations including amplifications and homozygous deletions in a clinical grade, regulatory agency approved (CLIA/CAP) format using routine clinical FFPE samples
  • Know how to preserve and prepare surgical pathology and cytologic samples including fluids and fine needle aspirations for NGS testing
  • Contrast the uses and limitations of using a blood sample for analysis of circulating tumor cell and cell free DNA for NGS
  • Be familiar with the potential of NGS to alter a cancer patient’s clinical outcome by identifying a genomic alteration that can lead to a specific targeted therapy that would not have been considered if the NGS results had not been available
  • Have a basic understanding of assessing quality assurance, proficiency testing and general validation of NGS procedures used in clinical testing of patient samples

Agenda

8:00 AM

Introductory Lectures: History of DNA Sequencing, Traditional and NGS Sequencing Technologies and the Roles of Pathologists in the Providing of NGS Results Designed to Direct Personalized Cancer Treatment

9:20 AM

Case 1: Non-small Cell Lung Cancer

9:40 AM

Case 2: Breast Cancer

10:00 AM

Break

10:20 AM

Case 3: Melanoma

10:40 AM

Case 4: Colorectal Cancer

11:00 AM

Case 5: Cancer of Unknown Primary Origin

11:20 AM

Case 6: Brain Tumors

11:40 AM

Questions and Discussion


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